Identification of o^-Adrenergic Receptors on Sarcolemma From Normal Subjects and Patients With Idiopathic Dilated Cardiomyopathy: Characteristics and Linkage to GTP-Binding Protein

Discontinuous density sucrose gradient centrifugation was used to isolate membrane vesicles from the left ventricle of three normal subjects (one prospective organ donor and two traffic victims whose hearts were obtained 1 hour after death) and nine patients undergoing cardiac transplantation as a consequence of idiopathic dilated cardiomyopathy. Sarcolemma-enriched subcellular fractions, detected in the interface between 8.55% and 25% sucrose, were identified by the increased activity of Na,K-ATPase and by enrichment in £-adrenergic receptor density. The density of /3-adrenergic receptors was lower in vesicles from diseased hearts (610±71 fmol/mg protein) than in vesicles from normal hearts (l,410±226 fmol/mg protein; p<0.01). a,-Adrenergic receptors were identified in these membrane vesicles by [H]prazosin binding. Specific binding of [H]prazosin was about 50% of the total binding at 1 nM, and a,-adrenergic binding sites were saturable at approximately 3 nM. Scatchard analysis revealed 58±5 fmol/mg protein (tfD=0.90±0.08 nM) in pathological hearts and 30±5 fmol/mg protein (A^D=0.90±0.03 nM) in normal hearts (p<0.01). The displacement curve of (-)-norepinephrine in membrane vesicles from normal hearts delineated one subpopulation of a,-adrenergic receptors; the addition of 0.1 mM GTP did not cause right shift. In membrane vesicles from diseased heart, the displacement curve of (-)-norepinephrine disclosed two subpopulations of a,-adrenergic receptors. A right shift that occurred after addition of GTP showed that in this case a,-adrenergic receptors were functionally coupled with GTP-binding protein. This study demonstrated the presence of a,-adrenergic receptors hi sarcolemma-enriched subcellular fractions from both normal and idiopathic dilated cardiomyopathic hearts. Only a,-adrenergic receptors from pathological hearts were coupled to GTP-binding protein. (Circulation Research 1989;64:474-481)